Composite

Part:BBa_K5061031

Designed by: Florent Cha   Group: iGEM24_Evry-Paris-Saclay   (2024-09-14)


Helper phage (HP) M13KO7-ΔgVI Golden Gate cloning platform

to do

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 4976
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 35
    Illegal NheI site found at 58
    Illegal PstI site found at 4976
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 12
    Illegal BamHI site found at 7785
    Illegal XhoI site found at 4819
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 4976
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 4976
    Illegal NgoMIV site found at 2511
    Illegal AgeI site found at 2765
    Illegal AgeI site found at 3089
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 18
    Illegal BsaI.rc site found at 6


[edit]
Categories
Parameters
None